2023   03   en   p.06-12 A.M. Mammedzade, B.U. Gasimli,
Structural origin of silk nanoparticles and their stabilization


A naturally occurring biopolymer known as silk fibroin (SF) has several distinctive qualities that make it an ideal vehicle for the delivery of drugs and a variety of therapeutic agents. It has been demonstrated that drugs and biomolecules can be successfully delivered via SF matrices. In this study homogenous SF nanoparticles (SFNP) obtained with a size ranging between 171-207 nm. SFNPs were stabilized with GA (glutaraldehyde) which is known as a cross-linking agent was used to stabilize the SFNPs. The stabilized SFNPs were consistent for 4 days. Circular dichroism (CD) and Fourier-transform infrared spectroscopy (FTIR) studies revealed random-coil to beta structure transition with beta structure reaching up to 59 %. Observed birefringence of Congo red staining in a polarized microscope indicates beta amyloid formation. Thus, SFNPs composed of amyloid nanoparticles.

Keywords: silk fibroin, silk nanoparticles, cross linker, amyloids.
PACS: 87.64.−t; 87.64.Cc


Received: 20.07.2023


Institute of Biophysics, Ministry of Science and Education Republic of Azerbaijan Z. Khalilov, 117, Baku, AZ 1171
E-mail: mesimlia@mail.ru; basira.qasimli.usubali@bsu.edu.az

Graphics and Images


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